• <span id="inn7i"><optgroup id="inn7i"></optgroup></span>
    產品中心您現在的位置:首頁 > 產品展示 > 化學試劑 > ClickChemistryTools > 點擊化學標記蛋白捕獲試劑盒Click-&-Go Click Chemistry Capture Kit

    點擊化學標記蛋白捕獲試劑盒Click-&-Go Click Chemistry Capture Kit

    更新時間:2025-04-15

    簡要描述:

    產品介紹Click--Go®ClickChemistryCapture試劑盒為具有疊氮化物或炔烴標記的蛋白質以及相應的點擊樹脂的研究人員提供了共價捕獲標記蛋白質的方法

    產品介紹

    Click-&-Go® Click Chemistry Capture 試劑盒為具有疊氮化物或炔烴標記的蛋白質以及相應的點擊樹脂的研究人員提供了共價捕獲標記蛋白質的方法。一旦通過銅催化的點擊化學共價連接到樹脂上,珠子就可以以的嚴格性洗滌,幾乎消除了任何非特異性結合的蛋白質,從而產生高度富集的目的分子群體。該試劑盒包含特殊配方的組分,既能催化點擊標記反應,又能防止與炔烴或疊氮化物改性樹脂的非特異性結合。其中對應的富集介質,試劑盒里面不包括需要單獨訂購。使用者根據自己的實驗設計,蛋白標記的基團選擇反應對應的瓊脂糖。炔基標記的蛋白,選擇疊氮脂糖樹脂(貨號:1038-2),疊氮標記的蛋白,選擇炔基瓊脂糖樹脂(貨號:1032-2)。


    Product introduction

    Click-&-Go® Click Chemistry Capture Kit provides researchers who have proteins labeled with an azide or alkyne and the corresponding click resin with the means to covalently capture labeled proteins. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. The kit contains specially formulated components to both catalyze the click labeling reaction and prevent non-specific binding to alkyne- or azide-modified resin*.    * enrichment media is not provided.

    Click-&-Go®Click Chemistry Capture Kit provides researchers who have proteins labeled with an azide or alkyne and the corresponding click resin with the means to covalently capture labeled proteins. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. The kit contains specially formulated components to both catalyze the click labeling reaction and prevent non-specific binding to alkyne- or azide-modified resin.

    Captured proteins can be protease digested to yield a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis, or release from beads under appropriate cleavage conditions for downstream analysis.

    Click-&-Go® Click Chemistry Capture Kit is comparable non-cleavable agarose azide/alkyne, Dde, PC, and disulfide agarose resin.

    The biotin-free enrichment improves signal to noise by eliminating nonspecific binding and increasing selectivity, thus improving detection of low abundant proteins. This approach is fully compatible with widely used MS techniques including iTRAC and ICAT.

    The biotin-free, covalent capture approach is superior to biotin or lectin based enrichment approaches, and ideal for proteomics, biomarker discovery, posttranslational modification (PTM) analysis, and more.


    方法對比

    Schematic representation of pull-down workflows for biotin-streptavidin and Click-&-Go® enrichment Protocols

    1065-schematic

    留言框

    • 產品:

    • 您的單位:

    • 您的姓名:

    • 聯系電話:

    • 常用郵箱:

    • 省份:

    • 詳細地址:

    • 補充說明:

    • 驗證碼:

      請輸入計算結果(填寫阿拉伯數字),如:三加四=7
    靶點科技(北京)有限公司

    靶點科技(北京)有限公司

    地址:中關村生命科學園北清創意園2-4樓2層

    © 2025 版權所有:靶點科技(北京)有限公司  備案號:京ICP備18027329號-2  總訪問量:305311  站點地圖  技術支持:化工儀器網  管理登陸

    主站蜘蛛池模板: 亚洲国产成人久久精品动漫| 亚洲一区二区高清| 亚洲色图.com| 四虎影视成人永久免费观看视频 | 99久久99热精品免费观看国产| 在线观看亚洲天天一三视| 天堂亚洲免费视频| 中文字幕第13亚洲另类| 黄色视屏在线免费播放| 2022中文字字幕久亚洲| 成人免费一区二区三区| 国产亚洲一区二区精品| 四虎影视在线影院在线观看免费视频| 无码乱人伦一区二区亚洲一| 国产免费无码一区二区| 亚洲精品美女视频| 最近中文字幕mv免费高清电影| 亚洲国产精品无码久久98| 国产日产成人免费视频在线观看| 极品色天使在线婷婷天堂亚洲 | 丁香花在线观看免费观看| 亚洲精品精华液一区二区| 国产免费av一区二区三区| 精品无码国产污污污免费网站国产 | 国产精品亚洲综合久久| 国产又粗又长又硬免费视频| 老司机午夜性生免费福利| 久久亚洲高清观看| h视频在线观看免费网站| 亚洲欧洲免费无码| 亚洲综合色视频在线观看| 91av视频免费在线观看| 亚洲丰满熟女一区二区哦| 国产精品亚洲αv天堂无码| 久久中文字幕免费视频| 亚洲国产综合在线| 亚洲成av人片天堂网老年人| 无码专区AAAAAA免费视频| 亚洲а∨天堂久久精品9966| 亚洲午夜精品久久久久久浪潮| 最近中文字幕mv免费高清在线|